Solution Hybrid Selection Capture for the Recovery of Functional Full-Length Eukaryotic cDNAs From Complex Environmental Samples
Identifieur interne : 000104 ( France/Analysis ); précédent : 000103; suivant : 000105Solution Hybrid Selection Capture for the Recovery of Functional Full-Length Eukaryotic cDNAs From Complex Environmental Samples
Auteurs : Claudia Bragalini [Italie, France] ; Céline Ribière [France] ; Nicolas Parisot [France] ; Laurent Vallon [France] ; Elsa Prudent [France] ; Eric Peyretaillade [France] ; Mariangela Girlanda [France, Italie] ; Pierre Peyret [France] ; Roland Marmeisse [Italie, France] ; Patricia Luis [France]Source :
- DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes [ 1340-2838 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN complémentaire (), ADN complémentaire (génétique), ADN complémentaire (isolement et purification), ARN messager (), ARN messager (génétique), ARN messager (isolement et purification), Bases de données d'acides nucléiques, Cellules eucaryotes, Microbiologie du sol, Métagénome, Saccharomyces cerevisiae (génétique), Sol ().
- MESH :
- génétique : ADN complémentaire, ARN messager, Saccharomyces cerevisiae.
- isolement et purification : ADN complémentaire, ARN messager.
- ADN complémentaire, ARN messager, Bases de données d'acides nucléiques, Cellules eucaryotes, Microbiologie du sol, Métagénome, Sol.
English descriptors
- KwdEn :
- DNA, Complementary (chemistry), DNA, Complementary (genetics), DNA, Complementary (isolation & purification), Databases, Nucleic Acid, Eukaryotic Cells, Metagenome, RNA, Messenger (chemistry), RNA, Messenger (genetics), RNA, Messenger (isolation & purification), Saccharomyces cerevisiae (genetics), Soil (chemistry), Soil Microbiology.
- MESH :
- chemical , chemistry : DNA, Complementary, RNA, Messenger, Soil.
- chemical , genetics : DNA, Complementary, RNA, Messenger.
- chemical , isolation & purification : DNA, Complementary, RNA, Messenger.
- genetics : Saccharomyces cerevisiae.
- Databases, Nucleic Acid, Eukaryotic Cells, Metagenome, Soil Microbiology.
Abstract
Eukaryotic microbial communities play key functional roles in soil biology and potentially represent a rich source of natural products including biocatalysts. Culture-independent molecular methods are powerful tools to isolate functional genes from uncultured microorganisms. However, none of the methods used in environmental genomics allow for a rapid isolation of numerous functional genes from eukaryotic microbial communities. We developed an original adaptation of the solution hybrid selection (SHS) for an efficient recovery of functional complementary DNAs (cDNAs) synthesized from soil-extracted polyadenylated mRNAs. This protocol was tested on the Glycoside Hydrolase 11 gene family encoding
Url:
DOI: 10.1093/dnares/dsu030
PubMed: 25281543
PubMed Central: 4263301
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000C00
- to stream Pmc, to step Curation: 000C00
- to stream Pmc, to step Checkpoint: 000F72
- to stream PubMed, to step Corpus: 001825
- to stream PubMed, to step Curation: 001825
- to stream PubMed, to step Checkpoint: 001740
- to stream Ncbi, to step Merge: 000F23
- to stream Ncbi, to step Curation: 000F23
- to stream Ncbi, to step Checkpoint: 000F23
- to stream Main, to step Merge: 001A89
- to stream Main, to step Curation: 001A82
- to stream Main, to step Exploration: 001A82
- to stream France, to step Extraction: 000104
Links to Exploration step
PMC:4263301Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Solution Hybrid Selection Capture for the Recovery of Functional
Full-Length Eukaryotic cDNAs From Complex Environmental Samples</title><author><name sortKey="Bragalini, Claudia" sort="Bragalini, Claudia" uniqKey="Bragalini C" first="Claudia" last="Bragalini">Claudia Bragalini</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Department of Life Sciences and Systems Biology</addr-line>,<institution>University of Turin</institution>,<addr-line>viale Mattioli 25, Turin 10125</addr-line>,<country>Italy</country></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ribiere, Celine" sort="Ribiere, Celine" uniqKey="Ribiere C" first="Céline" last="Ribière">Céline Ribière</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Parisot, Nicolas" sort="Parisot, Nicolas" uniqKey="Parisot N" first="Nicolas" last="Parisot">Nicolas Parisot</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Vallon, Laurent" sort="Vallon, Laurent" uniqKey="Vallon L" first="Laurent" last="Vallon">Laurent Vallon</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Prudent, Elsa" sort="Prudent, Elsa" uniqKey="Prudent E" first="Elsa" last="Prudent">Elsa Prudent</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Peyretaillade, Eric" sort="Peyretaillade, Eric" uniqKey="Peyretaillade E" first="Eric" last="Peyretaillade">Eric Peyretaillade</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Girlanda, Mariangela" sort="Girlanda, Mariangela" uniqKey="Girlanda M" first="Mariangela" last="Girlanda">Mariangela Girlanda</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af4"><institution>Istituto per la Protezione Sostenibile delle Piante (IPSP), Consiglio Nazionale delle Ricerche</institution>,<addr-line>Viale Mattioli 25, Turin 10125</addr-line>,<country>Italy</country></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Peyret, Pierre" sort="Peyret, Pierre" uniqKey="Peyret P" first="Pierre" last="Peyret">Pierre Peyret</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Department of Life Sciences and Systems Biology</addr-line>,<institution>University of Turin</institution>,<addr-line>viale Mattioli 25, Turin 10125</addr-line>,<country>Italy</country></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Luis, Patricia" sort="Luis, Patricia" uniqKey="Luis P" first="Patricia" last="Luis">Patricia Luis</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25281543</idno><idno type="pmc">4263301</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263301</idno><idno type="RBID">PMC:4263301</idno><idno type="doi">10.1093/dnares/dsu030</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000C00</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C00</idno><idno type="wicri:Area/Pmc/Curation">000C00</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C00</idno><idno type="wicri:Area/Pmc/Checkpoint">000F72</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000F72</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:25281543</idno><idno type="wicri:Area/PubMed/Corpus">001825</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001825</idno><idno type="wicri:Area/PubMed/Curation">001825</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001825</idno><idno type="wicri:Area/PubMed/Checkpoint">001740</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001740</idno><idno type="wicri:Area/Ncbi/Merge">000F23</idno><idno type="wicri:Area/Ncbi/Curation">000F23</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F23</idno><idno type="wicri:doubleKey">1340-2838:2014:Bragalini C:solution:hybrid:selection</idno><idno type="wicri:Area/Main/Merge">001A89</idno><idno type="wicri:Area/Main/Curation">001A82</idno><idno type="wicri:Area/Main/Exploration">001A82</idno><idno type="wicri:Area/France/Extraction">000104</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Solution Hybrid Selection Capture for the Recovery of Functional
Full-Length Eukaryotic cDNAs From Complex Environmental Samples</title><author><name sortKey="Bragalini, Claudia" sort="Bragalini, Claudia" uniqKey="Bragalini C" first="Claudia" last="Bragalini">Claudia Bragalini</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Department of Life Sciences and Systems Biology</addr-line>,<institution>University of Turin</institution>,<addr-line>viale Mattioli 25, Turin 10125</addr-line>,<country>Italy</country></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ribiere, Celine" sort="Ribiere, Celine" uniqKey="Ribiere C" first="Céline" last="Ribière">Céline Ribière</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Parisot, Nicolas" sort="Parisot, Nicolas" uniqKey="Parisot N" first="Nicolas" last="Parisot">Nicolas Parisot</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Vallon, Laurent" sort="Vallon, Laurent" uniqKey="Vallon L" first="Laurent" last="Vallon">Laurent Vallon</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Prudent, Elsa" sort="Prudent, Elsa" uniqKey="Prudent E" first="Elsa" last="Prudent">Elsa Prudent</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Peyretaillade, Eric" sort="Peyretaillade, Eric" uniqKey="Peyretaillade E" first="Eric" last="Peyretaillade">Eric Peyretaillade</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Girlanda, Mariangela" sort="Girlanda, Mariangela" uniqKey="Girlanda M" first="Mariangela" last="Girlanda">Mariangela Girlanda</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af4"><institution>Istituto per la Protezione Sostenibile delle Piante (IPSP), Consiglio Nazionale delle Ricerche</institution>,<addr-line>Viale Mattioli 25, Turin 10125</addr-line>,<country>Italy</country></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Peyret, Pierre" sort="Peyret, Pierre" uniqKey="Peyret P" first="Pierre" last="Peyret">Pierre Peyret</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>EA 4678 CIDAM, BP 10448</addr-line>,<institution>Clermont Université, Université d'Auvergne</institution>,<addr-line>Clermont-Ferrand F-63001</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Department of Life Sciences and Systems Biology</addr-line>,<institution>University of Turin</institution>,<addr-line>viale Mattioli 25, Turin 10125</addr-line>,<country>Italy</country></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Luis, Patricia" sort="Luis, Patricia" uniqKey="Luis P" first="Patricia" last="Luis">Patricia Luis</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Ecologie Microbienne, UMR CNRS 5557, USC INRA 1364</addr-line>,<institution>Université de Lyon, Université Lyon 1</institution>,<addr-line>Villeurbanne 69622</addr-line>,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes</title><idno type="ISSN">1340-2838</idno><idno type="eISSN">1756-1663</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (isolation & purification)</term><term>Databases, Nucleic Acid</term><term>Eukaryotic Cells</term><term>Metagenome</term><term>RNA, Messenger (chemistry)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (isolation & purification)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Soil (chemistry)</term><term>Soil Microbiology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire ()</term><term>ADN complémentaire (génétique)</term><term>ADN complémentaire (isolement et purification)</term><term>ARN messager ()</term><term>ARN messager (génétique)</term><term>ARN messager (isolement et purification)</term><term>Bases de données d'acides nucléiques</term><term>Cellules eucaryotes</term><term>Microbiologie du sol</term><term>Métagénome</term><term>Saccharomyces cerevisiae (génétique)</term><term>Sol ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term><term>RNA, Messenger</term><term>Soil</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ARN messager</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term><term>ARN messager</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Databases, Nucleic Acid</term><term>Eukaryotic Cells</term><term>Metagenome</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>ARN messager</term><term>Bases de données d'acides nucléiques</term><term>Cellules eucaryotes</term><term>Microbiologie du sol</term><term>Métagénome</term><term>Sol</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Eukaryotic microbial communities play key functional roles in soil biology and potentially represent a rich source of natural products including biocatalysts. Culture-independent molecular methods are powerful tools to isolate functional genes from uncultured microorganisms. However, none of the methods used in environmental genomics allow for a rapid isolation of numerous functional genes from eukaryotic microbial communities. We developed an original adaptation of the solution hybrid selection (SHS) for an efficient recovery of functional complementary DNAs (cDNAs) synthesized from soil-extracted polyadenylated mRNAs. This protocol was tested on the Glycoside Hydrolase 11 gene family encoding <italic>endo</italic>-xylanases for which we designed 35 explorative 31-mers capture probes. SHS was implemented on four soil eukaryotic cDNA pools. After two successive rounds of capture, >90% of the resulting cDNAs were GH11 sequences, of which 70% (38 among 53 sequenced genes) were full length. Between 1.5 and 25% of the cloned captured sequences were expressed in <italic>Saccharomyces cerevisiae</italic>. Sequencing of polymerase chain reaction-amplified GH11 gene fragments from the captured sequences highlighted hundreds of phylogenetically diverse sequences that were not yet described, in public databases. This protocol offers the possibility of performing exhaustive exploration of eukaryotic gene families within microbial communities thriving in any type of environment.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Tringe, S G" uniqKey="Tringe S">S.G. Tringe</name></author><author><name sortKey="Rubin, E M" uniqKey="Rubin E">E.M. Rubin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simon, C" uniqKey="Simon C">C. Simon</name></author><author><name sortKey="Daniel, R" uniqKey="Daniel R">R. Daniel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Howe, A C" uniqKey="Howe A">A.C. Howe</name></author><author><name sortKey="Jansson, J K" uniqKey="Jansson J">J.K. Jansson</name></author><author><name sortKey="Malfatti, S A" uniqKey="Malfatti S">S.A. Malfatti</name></author><author><name sortKey="Tringe, S G" uniqKey="Tringe S">S.G. Tringe</name></author><author><name sortKey="Tiedje, J M" uniqKey="Tiedje J">J.M. Tiedje</name></author><author><name sortKey="Brown, C T" uniqKey="Brown C">C.T. Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vo Kova, J" uniqKey="Vo Kova J">J. Voříšková</name></author><author><name sortKey="Baldrian, P" uniqKey="Baldrian P">P. Baldrian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luis, P" uniqKey="Luis P">P. Luis</name></author><author><name sortKey="Kellner, H" uniqKey="Kellner H">H. Kellner</name></author><author><name sortKey="Zimdars, B" uniqKey="Zimdars B">B. Zimdars</name></author><author><name sortKey="Langer, U" uniqKey="Langer U">U. Langer</name></author><author><name sortKey="Martin, F" uniqKey="Martin F">F. Martin</name></author><author><name sortKey="Buscot, F" uniqKey="Buscot F">F. Buscot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kellner, H" uniqKey="Kellner H">H. Kellner</name></author><author><name sortKey="Luis, P" uniqKey="Luis P">P. Luis</name></author><author><name sortKey="Pecyna, M J" uniqKey="Pecyna M">M.J. Pecyna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kellner, H" uniqKey="Kellner H">H. Kellner</name></author><author><name sortKey="Zak, D R" uniqKey="Zak D">D.R. Zak</name></author><author><name sortKey="Vandenbol, M" uniqKey="Vandenbol M">M. Vandenbol</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hong, S" uniqKey="Hong S">S. Hong</name></author><author><name sortKey="Bunge, J" uniqKey="Bunge J">J. Bunge</name></author><author><name sortKey="Leslin, C" uniqKey="Leslin C">C. Leslin</name></author><author><name sortKey="Jeon, S" uniqKey="Jeon S">S. Jeon</name></author><author><name sortKey="Epstein, S S" uniqKey="Epstein S">S.S. Epstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denonfoux, J" uniqKey="Denonfoux J">J. Denonfoux</name></author><author><name sortKey="Parisot, N" uniqKey="Parisot N">N. Parisot</name></author><author><name sortKey="Dugat Bony, E" uniqKey="Dugat Bony E">E. Dugat-Bony</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dugat Bony, E" uniqKey="Dugat Bony E">E. Dugat-Bony</name></author><author><name sortKey="Missaoui, M" uniqKey="Missaoui M">M. Missaoui</name></author><author><name sortKey="Peyretaillade, E" uniqKey="Peyretaillade E">E. Peyretaillade</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parisot, N" uniqKey="Parisot N">N. Parisot</name></author><author><name sortKey="Denonfoux, J" uniqKey="Denonfoux J">J. Denonfoux</name></author><author><name sortKey="Dugat Bony, E" uniqKey="Dugat Bony E">E. Dugat-Bony</name></author><author><name sortKey="Peyret, P" uniqKey="Peyret P">P. Peyret</name></author><author><name sortKey="Peyretaillade, E" uniqKey="Peyretaillade E">E. Peyretaillade</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bailly, J" uniqKey="Bailly J">J. Bailly</name></author><author><name sortKey="Fraissinet Tachet, L" uniqKey="Fraissinet Tachet L">L. Fraissinet-Tachet</name></author><author><name sortKey="Verner, M C" uniqKey="Verner M">M.C. Verner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kellner, H" uniqKey="Kellner H">H. Kellner</name></author><author><name sortKey="Luis, P" uniqKey="Luis P">P. Luis</name></author><author><name sortKey="Portetelle, D" uniqKey="Portetelle D">D. Portetelle</name></author><author><name sortKey="Vandenbol, M" uniqKey="Vandenbol M">M. Vandenbol</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Damon, C" uniqKey="Damon C">C. Damon</name></author><author><name sortKey="Vallon, L" uniqKey="Vallon L">L. Vallon</name></author><author><name sortKey="Zimmermann, S" uniqKey="Zimmermann S">S. Zimmermann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lehembre, F" uniqKey="Lehembre F">F. Lehembre</name></author><author><name sortKey="Doillon, D" uniqKey="Doillon D">D. Doillon</name></author><author><name sortKey="David, E" uniqKey="David E">E. David</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takasaki, K" uniqKey="Takasaki K">K. Takasaki</name></author><author><name sortKey="Miura, T" uniqKey="Miura T">T. Miura</name></author><author><name sortKey="Kanno, M" uniqKey="Kanno M">M. Kanno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bates, S T" uniqKey="Bates S">S.T. Bates</name></author><author><name sortKey="Clemente, J C" uniqKey="Clemente J">J.C. Clemente</name></author><author><name sortKey="Flores, G E" uniqKey="Flores G">G.E. Flores</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taylor, D L" uniqKey="Taylor D">D.L. Taylor</name></author><author><name sortKey="Hollingsworth, T N" uniqKey="Hollingsworth T">T.N. Hollingsworth</name></author><author><name sortKey="Mcfarland, J W" uniqKey="Mcfarland J">J.W. McFarland</name></author><author><name sortKey="Lennon, N J" uniqKey="Lennon N">N.J. Lennon</name></author><author><name sortKey="Nusbaum, C" uniqKey="Nusbaum C">C. Nusbaum</name></author><author><name sortKey="Ruess, R W" uniqKey="Ruess R">R.W. Ruess</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schneider, T" uniqKey="Schneider T">T. Schneider</name></author><author><name sortKey="Keiblinger, K M" uniqKey="Keiblinger K">K.M. Keiblinger</name></author><author><name sortKey="Schmid, E" uniqKey="Schmid E">E. Schmid</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stursova, M" uniqKey="Stursova M">M. Stursová</name></author><author><name sortKey="Zif Kova, L" uniqKey="Zif Kova L">L. Zifčáková</name></author><author><name sortKey="Leigh, M B" uniqKey="Leigh M">M.B. Leigh</name></author><author><name sortKey="Burgess, R" uniqKey="Burgess R">R. Burgess</name></author><author><name sortKey="Baldrian, P" uniqKey="Baldrian P">P. Baldrian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Demain, A L" uniqKey="Demain A">A.L. Demain</name></author><author><name sortKey="Dana, C A" uniqKey="Dana C">C.A. Dana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Damon, C" uniqKey="Damon C">C. Damon</name></author><author><name sortKey="Lehembre, F" uniqKey="Lehembre F">F. Lehembre</name></author><author><name sortKey="Oger Desfeux, C" uniqKey="Oger Desfeux C">C. Oger-Desfeux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grigoriev, I V" uniqKey="Grigoriev I">I.V. Grigoriev</name></author><author><name sortKey="Nikitin, R" uniqKey="Nikitin R">R. Nikitin</name></author><author><name sortKey="Haridas, S" uniqKey="Haridas S">S. Haridas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lombard, V" uniqKey="Lombard V">V. Lombard</name></author><author><name sortKey="Golaconda Ramulu, H" uniqKey="Golaconda Ramulu H">H. Golaconda Ramulu</name></author><author><name sortKey="Drula, E" uniqKey="Drula E">E. Drula</name></author><author><name sortKey="Coutinho, P M" uniqKey="Coutinho P">P.M. Coutinho</name></author><author><name sortKey="Henrissat, B" uniqKey="Henrissat B">B. Henrissat</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Margesin, R" uniqKey="Margesin R">R. Margesin</name></author><author><name sortKey="Minerbi, S" uniqKey="Minerbi S">S. Minerbi</name></author><author><name sortKey="Schinner, F" uniqKey="Schinner F">F. Schinner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paes, G" uniqKey="Paes G">G. Paës</name></author><author><name sortKey="Berrin, J G" uniqKey="Berrin J">J.G. Berrin</name></author><author><name sortKey="Beaugrand, J" uniqKey="Beaugrand J">J. Beaugrand</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuramae, E E" uniqKey="Kuramae E">E.E. Kuramae</name></author><author><name sortKey="Hillekens, R H" uniqKey="Hillekens R">R.H. Hillekens</name></author><author><name sortKey="De Hollander, M" uniqKey="De Hollander M">M. de Hollander</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luis, P" uniqKey="Luis P">P. Luis</name></author><author><name sortKey="Kellner, H" uniqKey="Kellner H">H. Kellner</name></author><author><name sortKey="Martin, F" uniqKey="Martin F">F. Martin</name></author><author><name sortKey="Buscot, F" uniqKey="Buscot F">F. Buscot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Damon, C" uniqKey="Damon C">C. Damon</name></author><author><name sortKey="Barroso, G" uniqKey="Barroso G">G. Barroso</name></author><author><name sortKey="Ferandon, C" uniqKey="Ferandon C">C. Férandon</name></author><author><name sortKey="Ranger, J" uniqKey="Ranger J">J. Ranger</name></author><author><name sortKey="Fraissinet Tachet, L" uniqKey="Fraissinet Tachet L">L. Fraissinet-Tachet</name></author><author><name sortKey="Marmeisse, R" uniqKey="Marmeisse R">R. Marmeisse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Altschul, S F" uniqKey="Altschul S">S.F. Altschul</name></author><author><name sortKey="Gish, W" uniqKey="Gish W">W. Gish</name></author><author><name sortKey="Miller, W" uniqKey="Miller W">W. Miller</name></author><author><name sortKey="Myers, E W" uniqKey="Myers E">E.W. Myers</name></author><author><name sortKey="Lipman, D J" uniqKey="Lipman D">D.J. Lipman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gnirke, A" uniqKey="Gnirke A">A. Gnirke</name></author><author><name sortKey="Melnikov, A" uniqKey="Melnikov A">A. Melnikov</name></author><author><name sortKey="Maguire, J" uniqKey="Maguire J">J. Maguire</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Masella, A P" uniqKey="Masella A">A.P. Masella</name></author><author><name sortKey="Bartram, A K" uniqKey="Bartram A">A.K. Bartram</name></author><author><name sortKey="Truszkowski, J M" uniqKey="Truszkowski J">J.M. Truszkowski</name></author><author><name sortKey="Brown, D G" uniqKey="Brown D">D.G. Brown</name></author><author><name sortKey="Neufeld, J D" uniqKey="Neufeld J">J.D. Neufeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schloss, P D" uniqKey="Schloss P">P.D. Schloss</name></author><author><name sortKey="Westcott, S L" uniqKey="Westcott S">S.L. Westcott</name></author><author><name sortKey="Ryabin, T" uniqKey="Ryabin T">T. Ryabin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Edgar, R C" uniqKey="Edgar R">R.C. Edgar</name></author><author><name sortKey="Haas, B J" uniqKey="Haas B">B.J. Haas</name></author><author><name sortKey="Clemente, J C" uniqKey="Clemente J">J.C. Clemente</name></author><author><name sortKey="Quince, C" uniqKey="Quince C">C. Quince</name></author><author><name sortKey="Knight, R" uniqKey="Knight R">R. Knight</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stothard, P" uniqKey="Stothard P">P. Stothard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rentsch, D" uniqKey="Rentsch D">D. Rentsch</name></author><author><name sortKey="Laloi, M" uniqKey="Laloi M">M. Laloi</name></author><author><name sortKey="Rouhara, I" uniqKey="Rouhara I">I. Rouhara</name></author><author><name sortKey="Schmelzer, E" uniqKey="Schmelzer E">E. Schmelzer</name></author><author><name sortKey="Delrot, S" uniqKey="Delrot S">S. Delrot</name></author><author><name sortKey="Frommer, W B" uniqKey="Frommer W">W.B. Frommer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J. Sambrook</name></author><author><name sortKey="Russell, D W" uniqKey="Russell D">D.W. Russell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rose, M" uniqKey="Rose M">M. Rose</name></author><author><name sortKey="Winston, F" uniqKey="Winston F">F. Winston</name></author><author><name sortKey="Hieter, P" uniqKey="Hieter P">P. Hieter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Edgar, R C" uniqKey="Edgar R">R.C. Edgar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gouy, M" uniqKey="Gouy M">M. Gouy</name></author><author><name sortKey="Guindon, S" uniqKey="Guindon S">S. Guindon</name></author><author><name sortKey="Gascuel, O" uniqKey="Gascuel O">O. Gascuel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K. Tamura</name></author><author><name sortKey="Stecher, G" uniqKey="Stecher G">G. Stecher</name></author><author><name sortKey="Peterson, D" uniqKey="Peterson D">D. Peterson</name></author><author><name sortKey="Filipski, A" uniqKey="Filipski A">A. Filipski</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S. Kumar</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>France</li><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Bragalini, Claudia" sort="Bragalini, Claudia" uniqKey="Bragalini C" first="Claudia" last="Bragalini">Claudia Bragalini</name></noRegion><name sortKey="Girlanda, Mariangela" sort="Girlanda, Mariangela" uniqKey="Girlanda M" first="Mariangela" last="Girlanda">Mariangela Girlanda</name><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name></country><country name="France"><noRegion><name sortKey="Bragalini, Claudia" sort="Bragalini, Claudia" uniqKey="Bragalini C" first="Claudia" last="Bragalini">Claudia Bragalini</name></noRegion><name sortKey="Girlanda, Mariangela" sort="Girlanda, Mariangela" uniqKey="Girlanda M" first="Mariangela" last="Girlanda">Mariangela Girlanda</name><name sortKey="Luis, Patricia" sort="Luis, Patricia" uniqKey="Luis P" first="Patricia" last="Luis">Patricia Luis</name><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name><name sortKey="Parisot, Nicolas" sort="Parisot, Nicolas" uniqKey="Parisot N" first="Nicolas" last="Parisot">Nicolas Parisot</name><name sortKey="Peyret, Pierre" sort="Peyret, Pierre" uniqKey="Peyret P" first="Pierre" last="Peyret">Pierre Peyret</name><name sortKey="Peyretaillade, Eric" sort="Peyretaillade, Eric" uniqKey="Peyretaillade E" first="Eric" last="Peyretaillade">Eric Peyretaillade</name><name sortKey="Prudent, Elsa" sort="Prudent, Elsa" uniqKey="Prudent E" first="Elsa" last="Prudent">Elsa Prudent</name><name sortKey="Ribiere, Celine" sort="Ribiere, Celine" uniqKey="Ribiere C" first="Céline" last="Ribière">Céline Ribière</name><name sortKey="Vallon, Laurent" sort="Vallon, Laurent" uniqKey="Vallon L" first="Laurent" last="Vallon">Laurent Vallon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000104 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000104 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= France
|étape= Analysis
|type= RBID
|clé= PMC:4263301
|texte= Solution Hybrid Selection Capture for the Recovery of Functional
Full-Length Eukaryotic cDNAs From Complex Environmental Samples
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/France/Analysis/RBID.i -Sk "pubmed:25281543" \
| HfdSelect -Kh $EXPLOR_AREA/Data/France/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |